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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a essential part in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two added splice variants have been reported but in-depth nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind especially into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged via the antibody.
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This manual outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the best possible final results are...
Antibodies certain to the blocking peptide no longer bind on the epitope about the focus on protein. By comparing the staining in the blocked antibody vs . the antibody on your own, one can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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